alpha Actinin antibody

Catalog No. ABIN7212180

The Mouse Monoclonal anti-alpha Actinin antibody has been validated for IHC (p). It is suitable to detect alpha Actinin in samples from Human, Mouse and Rat.

Quick Overview for alpha Actinin antibody (ABIN7212180)

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This alpha Actinin antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 4A12

Product Details anti-alpha Actinin Antibody

Purpose: a-actinin Monoclonal Antibody (4A12)

Specificity: A-actinin Monoclonal Antibody(4A12) detects endogenous levels of ACTN1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen

Immunogen: Recombinant Protein of a-actinin of ACTN1

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:200).

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for alpha Actinin

Target: alpha Actinin (ACTN) (alpha-Actinin Skeletal Muscle (ACTN))

Alternative Name: a-actinin

Background: Mouse Anti-a-actinin Monoclonal Antibody (4A12),ACTN1,Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. ACTN1 (actinin alpha 1) encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for ACTN1.,ACTN1

Gene ID: 87

UniProt: P12814

Pathways: Cell-Cell Junction Organization, Negative Regulation of Transporter Activity