Aminoacylase 1 antibody

Catalog No. ABIN7227208

The Rabbit Polyclonal anti-Aminoacylase 1 antibody (ABIN7227208) specifically detects Aminoacylase 1 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for Aminoacylase 1 antibody (ABIN7227208)

Target: Aminoacylase 1 (ACY1)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Aminoacylase 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Aminoacylase 1 Antibody

Purpose: Rabbit Anti-ACY1 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of ACY1 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ACY1 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Aminoacylase 1

Target: Aminoacylase 1 (ACY1)

Alternative Name: ACY1

Background: N-acyl-L-amino-acid amidohydrolaseACY1 encodes a cytosolic, homodimeric, zinc-binding enzyme that catalyzes the hydrolysis of acylated L-amino acids to L-amino acids and an acyl group, and has been postulated to function in the catabolism and salvage of acylated amino acids. ACY1 is located on chromosome 3p21. , a region reduced to homozygosity in small-cell lung cancer (SCLC), and its expression has been reported to be reduced or undetectable in SCLC cell lines and tumors. The amino acid sequence of human aminoacylase-1 is highly homologous to the porcine counterpart, and this enzyme is the first member of a new family of zinc-binding enzymes. Mutations in ACY1 cause aminoacylase-1 deficiency, a metabolic disorder characterized by central nervous system defects and increased urinary excretion of N-acetylated amino acids. Alternative splicing of this gene results in multiple transcript variants. Read-through transcription also exists between ACY1 and the upstream ABHD14A (abhydrolase domain containing 14A) gene, as represented in GeneID: 100526760. A related pseudogene has been identified on chromosome 18.

Molecular Weight: 44kD

Gene ID: 95

UniProt: Q03154