APC1 antibody

Catalog No. ABIN7224306

The Rabbit Polyclonal anti-APC1 antibody is suitable to detect APC1 in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for APC1 antibody (ABIN7224306)

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APC1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-APC1 Antibody

Purpose: Rabbit Anti-APC1 Polyclonal Antibody

Specificity: APC1 Polyclonal Antibody detects endogenous levels of APC1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human APC1 around the non-phosphorylation site of S688

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for APC1

Target: APC1 (ANAPC1) (Anaphase Promoting Complex Subunit 1 (ANAPC1))

Alternative Name: APC1

Background: ANAPC1, TSG24, Anaphase-promoting complex subunit 1, APC1, Cyclosome subunit 1, Mitotic checkpoint regulator, Testis-specific gene 24 proteinANAPC1 (anaphase promoting complex subunit 1) encodes a subunit of the anaphase-promoting complex. This complex is an E3 ubiquitin ligase that regulates progression through the metaphase to anaphase portion of the cell cycle by ubiquitinating proteins which targets them for degradation.

Gene ID: 64682

UniProt: Q9H1A4