ANKRD30A antibody (AA 1010-1090)

Catalog No. ABIN7213582

The Rabbit Polyclonal anti-ANKRD30A antibody is suitable to detect ANKRD30A in samples from Human. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for ANKRD30A antibody (AA 1010-1090) (ABIN7213582)

Target: ANKRD30A (Ankyrin Repeat Domain 30A (ANKRD30A))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ANKRD30A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ANKRD30A Antibody

Binding Specificity: AA 1010-1090

Purpose: Rabbit Anti-ANKRD30A Polyclonal Antibody

Specificity: ANKRD30A Polyclonal Antibody detects endogenous levels of ANKRD30A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human ANKRD30A at AA range: 1010-1090

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ANKRD30A

Target: ANKRD30A (Ankyrin Repeat Domain 30A (ANKRD30A))

Alternative Name: ANKRD30A

Background: ANKRD30A, Ankyrin repeat domain-containing protein 30A, Serologically defined breast cancer antigen NY-BR-1Ankyrins are membrane adaptor molecules that play important roles in coupling integral membrane proteins to the spectrin-based cytoskeleton network. Mutations of ankyrin genes lead to severe genetic diseases, such as fatal cardiac arrhythmias and hereditary spherocytosis. ANKRD30A (ankyrin repeat domain 30A), also known as serologically defined breast cancer antigen NY-BR-1, is a 1,397 amino acid coiled-coil protein. Containing six ANK repeats and thirty-seven exons, ANKRD30A is a distant homolog of the POTE family. Initiated by either Dnmt1 or Met-57, ANKRD30A is highly expressed in breast and testis, and faintly in placenta. Present in 80 % of breast cancers, but not in other types of tumors, ANKRD30A is a differentiation antigen and a potential target for antibody-based therapies in breast cancers.

Molecular Weight: 153kD

Gene ID: 91074

UniProt: Q9BXX3