Ki-67 antibody (AA 3170-3250)

Catalog No. ABIN7215534

This anti-Ki-67 antibody is a Rabbit Polyclonal antibody detecting Ki-67 in WB, IF, ELISA and IHC (p). Suitable for Human.

Quick Overview for Ki-67 antibody (AA 3170-3250) (ABIN7215534)

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Ki-67 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Ki-67 Antibody

Binding Specificity: AA 3170-3250

Purpose: Ki-67 Polyclonal Antibody

Specificity: Ki-67 Polyclonal Antibody detects endogenous levels of Ki-67 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Ki-67 at AA range: 3170-3250

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Ki-67

Target: Ki-67 (MKI67) (Antigen Identified By Monoclonal Antibody Ki-67 (MKI67))

Alternative Name: Ki-67

Background: Rabbit Anti-Ki-67 Polyclonal Antibody,MKI67, Antigen KI-67,MKI67 encodes Antigen KI-67 that is associated with and may be necessary for cellular proliferation. Alternatively spliced transcript variants have been described. A related pseudogene exists on chromosome X.,Antigen KI-67

Molecular Weight: observerd band 360kDa

Gene ID: 4288

UniProt: P46013

Pathways: Glycosaminoglycan Metabolic Process