AP2 alpha/beta (AA 360-440) antibody

Catalog No. ABIN7213610

The Rabbit Polyclonal anti-AP2 alpha/beta antibody is suitable to detect AP2 alpha/beta in samples from Human, Mouse and Rat. It has been validated for WB, IHC, IF and ELISA.

Quick Overview for AP2 alpha/beta (AA 360-440) antibody (ABIN7213610)

Target: AP2 alpha/beta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA

Product Details

Binding Specificity: AA 360-440

Purpose: Rabbit Anti-AP-2α/β Polyclonal Antibody

Specificity: AP-2α/β Polyclonal Antibody detects endogenous levels of AP-2α/β protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human AP-2alpha/beta at AA range: 360-440

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details

Target: AP2 alpha/beta

Alternative Name: AP-2alpha/beta

Background: TFAP2A, AP2TF, TFAP2, Transcription factor AP-2-alpha, AP2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2, AP-2, TFAP2B, Transcription factor AP-2-beta, AP2-beta, Activating enhancer-bindiTranscription factor AP-2 alpha encoded by TFAP2A is a transcription factor that binds the consensus sequence 5'-GCCNNNGGC-3'. The encoded protein functions as either a homodimer or as a heterodimer with similar family members. This protein activates the transcription of some genes while inhibiting the transcription of others. Defects in this gene are a cause of branchiooculofacial syndrome (BOFS). Three transcript variants encoding different isoforms have been found for TFAP2A.

Molecular Weight: 49kD

Gene ID: 7020

UniProt: P05549, Q92481