APAF1 antibody (AA 260-340)

Catalog No. ABIN7213614

This anti-APAF1 antibody is a Rabbit Polyclonal antibody detecting APAF1 in WB, ELISA, IF and IHC (p). Suitable for Human.

Quick Overview for APAF1 antibody (AA 260-340) (ABIN7213614)

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This APAF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-APAF1 Antibody

Binding Specificity: AA 260-340

Purpose: Apaf-1-ALT Polyclonal Antibody

Specificity: Apaf-1-ALT Polyclonal Antibody detects endogenous levels of Apaf-1-ALT protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the C-terminal region of human Apaf-1-ALT at AA range: 260-340

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), IF (1:200-1:1000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for APAF1

Target: APAF1 (Apoptotic Peptidase Activating Factor 1 (APAF1))

Alternative Name: Apaf-1-ALT

Background: Rabbit Anti-Apaf-1-ALT Polyclonal Antibody,APAF1-interacting protein, APIP, APIP2, CGI-29 protein, MMRP19, dJ179L10.2, likely ortholog of mouse monocyte macrophage 19,APAF1 encodes a cytoplasmic protein that initiates apoptosis. This protein (apoptotic peptidase activating factor 1) contains several copies of the WD-40 domain, a caspase recruitment domain (CARD), and an ATPase domain (NB-ARC). Upon binding cytochrome c and dATP, this protein forms an oligomeric apoptosome. The apoptosome binds and cleaves caspase 9 preproprotein, releasing its mature, activated form. Activated caspase 9 stimulates the subsequent caspase cascade that commits the cell to apoptosis. Alternative splicing results in several transcript variants encoding different isoforms.,Apoptotic protease-activating factor 1

Gene ID: 317

Pathways: p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis, Tube Formation, Positive Regulation of Endopeptidase Activity