beta Arrestin 1 antibody (pSer412)

Catalog No. ABIN7222284

The Rabbit Polyclonal anti-beta Arrestin 1 antibody (ABIN7222284) specifically detects beta Arrestin 1 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Monkey samples.

Quick Overview for beta Arrestin 1 antibody (pSer412) (ABIN7222284)

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Reactivity: Human, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This beta Arrestin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-beta Arrestin 1 Antibody

Binding Specificity: pSer412

Purpose: Rabbit Anti-Arrestin-β-1 (phospho Ser412) Polyclonal Antibody

Specificity: Phospho-Arrestin-β-1 (S412) Polyclonal Antibody detects endogenous levels of Arrestin-β-1 protein only when phosphorylated at S412.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Arrestin-beta-1 Phospho-Ser412

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for beta Arrestin 1

Target: beta Arrestin 1 (ARRB1) (Arrestin, beta 1 (ARRB1))

Alternative Name: Arrestin-beta-1

Background: ARRB1, ARR1, Beta-arrestin-1, Arrestin beta-1Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described.

Molecular Weight: 47kD

Gene ID: 408

UniProt: P49407

Pathways: Positive Regulation of Peptide Hormone Secretion, Nuclear Hormone Receptor Binding, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction