ARID1B antibody (AA 1340-1420)

Catalog No. ABIN7223778

The Rabbit Polyclonal anti-ARID1B antibody (ABIN7223778) specifically detects ARID1B in WB, IF, ELISA and IHC. The antibody is reactive with Human and Rat samples.

Quick Overview for ARID1B antibody (AA 1340-1420) (ABIN7223778)

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARID1B antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), ELISA, Immunohistochemistry (IHC)

Product Details anti-ARID1B Antibody

Binding Specificity: AA 1340-1420

Purpose: Rabbit Anti-BAF250b Polyclonal Antibody

Specificity: BAF250b Polyclonal Antibody detects endogenous levels of BAF250b protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human BAF250b at AA range: 1340-1420

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ARID1B

Target: ARID1B (AT Rich Interactive Domain 1B (SWI1-Like) (ARID1B))

Alternative Name: BAF250b

Background: ARID1B, BAF250B, DAN15, KIAA1235, OSA2, AT-rich interactive domain-containing protein 1B, ARID domain-containing protein 1B, BRG1-associated factor 250b, BAF250B, BRG1-binding protein hELD/OSA1, Osa homolog 2, hOsa2, p250RARID1B (AT-rich interaction domain 1B) encodes an AT-rich DNA interacting domain-containing protein. The encoded protein is a component of the SWI/SNF chromatin remodeling complex and may play a role in cell-cycle activation. The protein encoded by ARID1B is similar to AT-rich interactive domain-containing protein 1A. These two proteins function as alternative, mutually exclusive ARID-subunits of the SWI/SNF complex. The associated complexes play opposing roles. Alternatively spliced transcript variants encoding different isoforms have been described.

Molecular Weight: 170kD

Gene ID: 57492

UniProt: Q8NFD5