ATOH8 antibody

Catalog No. ABIN7227408

The Rabbit Polyclonal anti-ATOH8 antibody (ABIN7227408) specifically detects ATOH8 in WB and ELISA. The antibody is reactive with Human and Mouse samples.

Quick Overview for ATOH8 antibody (ABIN7227408)

Target: ATOH8 (Atonal Homolog 8 (ATOH8))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATOH8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-ATOH8 Antibody

Purpose: Rabbit Anti-ATOH8 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of ATOH8 protein

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human ATOH8 protein

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000,ELISA 1:5000-20000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS, 50 % glycerol, 0.05 % Proclin 300, 0.05 %BSA

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATOH8

Target: ATOH8 (Atonal Homolog 8 (ATOH8))

Alternative Name: ATOH8

Background: Protein atonal homolog 8, Class A basic helix-loop-helix protein 21, bHLHa21, Helix-loop-helix protein hATH-6, hATH6Transcription factor that binds a palindromic (canonical) core consensus DNA sequence 5-CANNTG- 3 known as an E-box element, possibly as a heterodimer with other bHLH proteins. Regulates endothelial cell proliferation, migration and tube-like structures formation. Modulates endothelial cell differentiation through NOS3. May be implicated in specification and differentiation of neuronal cell lineages in the brain. May participate in kidney development and may be involved in podocyte differentiation. During early embryonic development is involved in tissue-specific differentiation processes that are dependent on class II bHLH factors and namely modulates the differentiation program initiated by the pro-endocrine factor NEUROG3. During myogenesis, may play a role during the transition of myoblasts from the proliferative phase to the differentiation phase. Positively regulates HAMP transcription in two ways, firstly by acting directly on the HAMP promoter via E-boxes binding and indirectly through increased phosphorylation of SMAD protein complex. Repress NEUROG3-dependent gene activation in a gene-specific manner through at least two mechanisms, requires only either the sequestering of a general partner such as TCF3 through heterodimerization, either also requires binding of the bHLH domain to DNA via a basic motif.

Molecular Weight: 35kD

Gene ID: 84913

UniProt: Q96SQ7

Pathways: Regulation of Muscle Cell Differentiation