ATP5F1D antibody (AA 30-110)

Catalog No. ABIN7223008

The Rabbit Polyclonal anti-ATP5F1D antibody is suitable to detect ATP5F1D in samples from Human, Mouse and Rat. It has been validated for ELISA, IHC and IF.

Quick Overview for ATP5F1D antibody (AA 30-110) (ABIN7223008)

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATP5F1D antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-ATP5F1D Antibody

Binding Specificity: AA 30-110

Purpose: Rabbit Anti-ATP5D Polyclonal Antibody

Specificity: ATP5D Polyclonal Antibody detects endogenous levels of ATP5D protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human ATP5D at AA range: 30-110

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for ATP5F1D

Target: ATP5F1D (ATP synthase subunit delta, mitochondrial (ATP5F1D))

Alternative Name: ATP5D

Background: ATP5D, ATP synthase subunit delta, mitochondrial, F-ATPase delta subunitATP5D (ATP synthase, H+ transporting, mitochondrial F1 complex, delta subunit) encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). ATP5D encodes the delta subunit of the catalytic core. Alternatively spliced transcript variants encoding the same isoform have been identified.

Gene ID: 513

UniProt: P30049

Pathways: Proton Transport, Ribonucleoside Biosynthetic Process