ATR antibody

Catalog No. ABIN7223284

The Rabbit Polyclonal anti-ATR antibody (ABIN7223284) specifically detects ATR in ELISA, WB and IHC (p). The antibody is reactive with Human samples.

Quick Overview for ATR antibody (ABIN7223284)

Target: ATR (ATR serine/threonine kinase (ATR))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATR antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ATR Antibody

Purpose: ATR Polyclonal Antibody

Specificity: ATR Polyclonal Antibody detects endogenous levels of ATR protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human ATR around the non-phosphorylation site of S428

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:40000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for ATR

Target: ATR (ATR serine/threonine kinase (ATR))

Alternative Name: ATR

Background: Rabbit Anti-ATR Polyclonal Antibody,ATR, FRP1, Serine/threonine-protein kinase ATR, Ataxia telangiectasia and Rad3-related protein, FRAP-related protein 1,The protein encoded by ATR (ATR serine/threonine kinase) belongs the PI3/PI4-kinase family, and is most closely related to ATM, a protein kinase encoded by the gene mutated in ataxia telangiectasia. This protein and ATM share similarity with Schizosaccharomyces pombe rad3, a cell cycle checkpoint gene required for cell cycle arrest and DNA damage repair in response to DNA damage. This kinase has been shown to phosphorylate checkpoint kinase CHK1, checkpoint proteins RAD17, and RAD9, as well as tumor suppressor protein BRCA1. Mutations of ATR are associated with Seckel syndrome. An alternatively spliced transcript variant of this gene has been reported, however, its full length nature is not known. Transcript variants utilizing alternative polyA sites exist.,serine threonine-protein kinase ATR

Gene ID: 545

UniProt: Q13535

Pathways: Positive Regulation of Response to DNA Damage Stimulus