AIRE antibody (pSer156)

Catalog No. ABIN7221558

The Rabbit Polyclonal anti-AIRE antibody (ABIN7221558) specifically detects AIRE in WB and ELISA. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for AIRE antibody (pSer156) (ABIN7221558)

Target: AIRE (Autoimmune Regulator (AIRE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This AIRE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-AIRE Antibody

Binding Specificity: pSer156

Purpose: Rabbit Anti-AIRE-1 (phospho Ser156) Polyclonal Antibody

Specificity: Phospho-AIRE-1 (S156) Polyclonal Antibody detects endogenous levels of AIRE-1 protein only when phosphorylated at S156.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human AIRE-1 Phospho-Ser156

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for AIRE

Target: AIRE (Autoimmune Regulator (AIRE))

Alternative Name: AIRE-1

Background: AIRE, APECED, Autoimmune regulator, Autoimmune polyendocrinopathy candidiasis ectodermal dystrophy protein, APECED proteinAIRE encodes a transcriptional regulator that forms nuclear bodies and interacts with the transcriptional coactivator CREB binding protein. The autoimmune regulator plays an important role in immunity by regulating the expression of autoantigens and negative selection of autoreactive T-cells in the thymus. Mutations in this gene cause the rare autosomal-recessive systemic autoimmune disease termed autoimmune polyendocrinopathy with candidiasis and ectodermal dystrophy (APECED).

Molecular Weight: 50kD

Gene ID: 326

UniProt: O43918

Pathways: Chromatin Binding