BLK antibody (pTyr501)

Catalog No. ABIN7224208

This Rabbit Polyclonal antibody specifically detects BLK in ELISA and IHC (p). It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for BLK antibody (pTyr501) (ABIN7224208)

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BLK antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-BLK Antibody

Binding Specificity: pTyr501

Purpose: Blk (phospho Tyr501) Polyclonal Antibody

Specificity: Phospho-Blk (Y501) Polyclonal Antibody detects endogenous levels of Blk protein only when phosphorylated at Y501.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Blk Phospho-Tyr501

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC-P (1:100-1:300), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BLK

Target: BLK (B Lymphoid Tyrosine Kinase (BLK))

Alternative Name: Blk

Background: Rabbit Anti-Blk (phospho Tyr501) Polyclonal Antibody,BLK, Tyrosine-protein kinase Blk, B lymphocyte kinase, p55-Blk,BLK (BLK proto-oncogene, Src family tyrosine kinase) encodes a nonreceptor tyrosine-kinase of the src family of proto-oncogenes that are typically involved in cell proliferation and differentiation. The protein has a role in B-cell receptor signaling and B-cell development. The protein also stimulates insulin synthesis and secretion in response to glucose and enhances the expression of several pancreatic beta-cell transcription factors.,Tyrosine-protein kinase Blk

Gene ID: 640

UniProt: P51451

Pathways: Positive Regulation of Peptide Hormone Secretion, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling