BCL6 antibody (pSer333)

Catalog No. ABIN7224024

The Rabbit Polyclonal anti-BCL6 antibody has been validated for WB and ELISA. It is suitable to detect BCL6 in samples from Human, Mouse and Rat.

Quick Overview for BCL6 antibody (pSer333) (ABIN7224024)

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCL6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-BCL6 Antibody

Binding Specificity: pSer333

Purpose: Bcl-6 (phospho Ser333) Polyclonal Antibody

Specificity: Phospho-Bcl-6 (S333) Polyclonal Antibody detects endogenous levels of Bcl-6 protein only when phosphorylated at S333.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Bcl-6 Phospho-Ser333

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for BCL6

Target: BCL6 (B-Cell CLL/lymphoma 6 (BCL6))

Alternative Name: Bcl-6

Background: Rabbit Anti-Bcl-6 (phospho Ser333) Polyclonal Antibody,BCL6, BCL5, LAZ3, ZBTB27, ZNF51, B-cell lymphoma 6 protein, BCL-6, B-cell lymphoma 5 protein, BCL-5, Protein LAZ-3, Zinc finger and BTB domain-containing protein 27, Zinc finger protein 51,The protein encoded by BCL6 (B-cell CLL/lymphoma 6) is a zinc finger transcription factor and contains an N-terminal POZ domain. This protein acts as a sequence-specific repressor of transcription, and has been shown to modulate the transcription of STAT-dependent IL-4 responses of B cells. This protein can interact with a variety of POZ-containing proteins that function as transcription corepressors. BCL6 is found to be frequently translocated and hypermutated in diffuse large-cell lymphoma (DLCL), and may be involved in the pathogenesis of DLCL. Alternatively spliced transcript variants encoding different protein isoforms have been found for BCL6.,B-cell lymphoma 6 protein

Gene ID: 604

UniProt: P41182

Pathways: Chromatin Binding, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus