B-Cell Linker antibody

Catalog No. ABIN7213812

This Rabbit Polyclonal antibody specifically detects B-Cell Linker in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for B-Cell Linker antibody (ABIN7213812)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-B-Cell Linker Antibody

Purpose: Rabbit Anti-BLNK Polyclonal Antibody

Specificity: BLNK Polyclonal Antibody detects endogenous levels of BLNK protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human BLNK around the non-phosphorylation site of Y96

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: BLNK, BASH, SLP65, B-cell linker protein, B-cell adapter containing a SH2 domain protein, B-cell adapter containing a Src homology 2 domain protein, Cytoplasmic adapter protein, Src homology 2 domain-containing leukocyte protein of 65 kDa,BLNK encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein (B-cell linker)has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants have been found for BLNK.

Molecular Weight: 50kD

Gene ID: 29760

UniProt: Q8WV28

Pathways: BCR Signaling