BAG4 antibody (C-Term)

Catalog No. ABIN7213758

The Rabbit Polyclonal anti-BAG4 antibody is suitable to detect BAG4 in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for BAG4 antibody (C-Term) (ABIN7213758)

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BAG4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BAG4 Antibody

Binding Specificity: C-Term

Purpose: Rabbit Anti-Bag-4 Polyclonal Antibody

Specificity: Bag-4 Polyclonal Antibody detects endogenous levels of Bag-4 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Bag-4

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BAG4

Target: BAG4 (BCL2-Associated Athanogene 4 (BAG4))

Alternative Name: Bag-4

Background: BAG4, SODD, BAG family molecular chaperone regulator 4, BAG-4, Bcl-2-associated athanogene 4, Silencer of death domainsThe protein encoded by BAG4 is a member of the BAG1-related protein family. BAG1 is an anti-apoptotic protein that functions through interactions with a variety of cell apoptosis and growth related proteins including BCL-2, Raf-protein kinase, steroid hormone receptors, growth factor receptors and members of the heat shock protein 70 kDa family. This protein contains a BAG domain near the C-terminus, which could bind and inhibit the chaperone activity of Hsc70/Hsp70. This protein (BCL2 associated athanogene 4) was found to be associated with the death domain of tumor necrosis factor receptor type 1 (TNF-R1) and death receptor-3 (DR3), and thereby negatively regulates downstream cell death signaling. The regulatory role of this protein in cell death was demonstrated in epithelial cells which undergo apoptosis while integrin mediated matrix contacts are lost. Alternatively spliced transcript variants encoding distinct isoforms have been identified.

Molecular Weight: 50kD

Gene ID: 9530

UniProt: O95429

Pathways: Regulation of Actin Filament Polymerization