BIK antibody (pThr33)

Catalog No. ABIN7224184

The Rabbit Polyclonal anti-BIK antibody (ABIN7224184) specifically detects BIK in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for BIK antibody (pThr33) (ABIN7224184)

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BIK antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-BIK Antibody

Binding Specificity: pThr33

Purpose: Rabbit Anti-NBK (phospho Thr33) Polyclonal Antibody

Specificity: Phospho-NBK (T33) Polyclonal Antibody detects endogenous levels of NBK protein only when phosphorylated at T33.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human NBK Phospho-Thr33

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BIK

Target: BIK (BCL2-Interacting Killer (Apoptosis-Inducing) (BIK))

Alternative Name: NBK

Background: BIK, NBK, Bcl-2-interacting killer, Apoptosis inducer NBK, BIP1, BP4The protein encoded by BIK (BCL2 interacting killer) shares a critical BH3 domain with other death-promoting proteins, such as BID, BAK, BAD and BAX, that is required for its pro-apoptotic activity, and for interaction with anti-apoptotic members of the BCL2 family, and viral survival-promoting proteins. Since the activity of this protein is suppressed in the presence of survival-promoting proteins, it is suggested as a likely target for anti-apoptotic proteins.

Molecular Weight: 30kD

Gene ID: 638

UniProt: Q13323

Pathways: Positive Regulation of Endopeptidase Activity