BNIP3 antibody (Internal Region)

Catalog No. ABIN7217856

The Rabbit Polyclonal anti-BNIP3 antibody (ABIN7217856) specifically detects BNIP3 in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for BNIP3 antibody (Internal Region) (ABIN7217856)

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-BNIP3 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-BNIP-3 Polyclonal Antibody

Specificity: BNIP-3 Polyclonal Antibody detects endogenous levels of BNIP-3 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human BNIP-3

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC: 1:100-300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BNIP3

Target: BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))

Alternative Name: BNIP-3

Background: BNIP3, NIP3, BCL2/adenovirus E1B 19 kDa protein-interacting protein 3BNIP3 is encodes a mitochondrial protein that contains a BH3 domain and acts as a pro-apoptotic factor. The BCL2 interacting protein 3 interacts with anti-apoptotic proteins, including the E1B 19 kDa protein and Bcl2. This gene is silenced in tumors by DNA methylation.

Molecular Weight: 22kD

Gene ID: 664

UniProt: Q12983

Pathways: Autophagy, Brown Fat Cell Differentiation