Beclin 1 antibody (AA 110-190)

Catalog No. ABIN7201595

This Mouse Monoclonal antibody specifically detects Beclin 1 in WB, IHC and IF. It exhibits reactivity toward Human, Mouse, Rat and Cow.

Quick Overview for Beclin 1 antibody (AA 110-190) (ABIN7201595)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human, Mouse, Rat, Cow

Host: Mouse

Clonality: Monoclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 5C2

Product Details anti-Beclin 1 Antibody

Binding Specificity: AA 110-190

Purpose: Mouse Anti-Beclin-1 Mouse Monoclonal Antibody (5C2)

Specificity: Beclin-1 protein detects endogenous levels of BECN1.

Purification: The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Immunogen: Synthetic Peptide of Beclin-1 at AA range of 110-190

Isotype: IgG1

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:1000-2000,IHC 1:100-200,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: Beclin-1

Background: BECN1BECN1 (beclin 1) encodes a protein that regulates autophagy, a catabolic process of degradation induced by starvation. The encoded protein is a component of the phosphatidylinositol-3-kinase (PI3K) complex which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. Alternative splicing results in multiple transcript variants.

Molecular Weight: 60kD

Gene ID: 8678

UniProt: Q14457

Pathways: Autophagy