BMX antibody (pTyr566)

Catalog No. ABIN7224400

The Rabbit Polyclonal anti-BMX antibody is suitable to detect BMX in samples from Human and Mouse. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for BMX antibody (pTyr566) (ABIN7224400)

Target: BMX (BMX Non-Receptor Tyrosine Kinase (BMX))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BMX antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-BMX Antibody

Binding Specificity: pTyr566

Purpose: Rabbit Anti-Bmx (phospho Tyr566) Polyclonal Antibody

Specificity: Phospho-Bmx (Y566) Polyclonal Antibody detects endogenous levels of Bmx protein only when phosphorylated at Y566.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Bmx Phospho-Tyr566

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BMX

Target: BMX (BMX Non-Receptor Tyrosine Kinase (BMX))

Alternative Name: Bmx

Background: BMX, Cytoplasmic tyrosine-protein kinase BMX, Bone marrow tyrosine kinase gene in chromosome X protein, Epithelial and endothelial tyrosine kinase, ETK, NTK38BMX (BMX non-receptor tyrosine kinase) encodes a non-receptor tyrosine kinase belonging to the Tec kinase family. The protein contains a PH-like domain, which mediates membrane targeting by binding to phosphatidylinositol 3,4,5-triphosphate (PIP3), and a SH2 domain that binds to tyrosine-phosphorylated proteins and functions in signal transduction. The protein is implicated in several signal transduction pathways including the Stat pathway, and regulates differentiation and tumorigenicity of several types of cancer cells. Alternatively spliced transcript variants have been found for BMX.

Molecular Weight: 78kD

Gene ID: 660

UniProt: P51813