BCR antibody

Catalog No. ABIN7213794

The Rabbit Polyclonal anti-BCR antibody (ABIN7213794) specifically detects BCR in WB, IHC, ELISA and IF. The antibody is reactive with Human and Mouse samples.

Quick Overview for BCR antibody (ABIN7213794)

Target: BCR (Breakpoint Cluster Region (BCR))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BCR antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-BCR Antibody

Purpose: Rabbit Anti-Bcr Polyclonal Antibody

Specificity: Bcr Polyclonal Antibody detects endogenous levels of Bcr protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Bcr around the non-phosphorylation site of Y177

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BCR

Target: BCR (Breakpoint Cluster Region (BCR))

Alternative Name: Bcr

Background: BCR, BCR1, D22S11, Breakpoint cluster region protein, Renal carcinoma antigen NY-REN-26A reciprocal translocation between chromosomes 22 and 9 produces the Philadelphia chromosome, which is often found in patients with chronic myelogenous leukemia. The chromosome 22 breakpoint for this translocation is located within the BCR gene. The translocation produces a fusion protein which is encoded by sequence from both BCR and ABL, the gene at the chromosome 9 breakpoint. Although the BCR-ABL fusion protein has been extensively studied, the function of the normal BCR gene product is not clear. BCR, RhoGEF and GTPase activating protein has serine/threonine kinase activity and is a GTPase-activating protein for p21rac. Two transcript variants encoding different isoforms have been found for BCR.

Molecular Weight: 143kD

Gene ID: 613

UniProt: P11274

Pathways: Regulation of Leukocyte Mediated Immunity, Platelet-derived growth Factor Receptor Signaling