BUB1 antibody (AA 220-300)

Catalog No. ABIN7224634

This Rabbit Polyclonal antibody specifically detects BUB1 in ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for BUB1 antibody (AA 220-300) (ABIN7224634)

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BUB1 antibody is un-conjugated

Application: ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-BUB1 Antibody

Binding Specificity: AA 220-300

Purpose: Rabbit Anti-Bub1 Polyclonal Antibody

Specificity: Bub1 Polyclonal Antibody detects endogenous levels of Bub1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Bub1 at AA range: 220-300

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,ELISA 1:10000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for BUB1

Target: BUB1 (Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast) (BUB1))

Alternative Name: Bub1

Background: BUB1, BUB1L, Mitotic checkpoint serine/threonine-protein kinase BUB1, hBUB1, BUB1ABUB1 (BUB1 mitotic checkpoint serine/threonine kinase) encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in BUB1 have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.

Gene ID: 699

UniProt: O43683