CAMK1 antibody (pThr177)

Catalog No. ABIN7225526

This Rabbit Polyclonal antibody specifically detects CAMK1 in WB, IHC, ELISA and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CAMK1 antibody (pThr177) (ABIN7225526)

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAMK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CAMK1 Antibody

Binding Specificity: pThr177

Purpose: Rabbit Anti-CaMKIα (phospho Thr177) Polyclonal Antibody

Specificity: Phospho-CaMKIα (T177) Polyclonal Antibody detects endogenous levels of CaMKIα protein only when phosphorylated at T177.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CaMKIalpha Phospho-Thr177

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CAMK1

Target: CAMK1 (Calcium/calmodulin-Dependent Protein Kinase I (CAMK1))

Alternative Name: CaMKIalpha

Background: CAMK1, Calcium/calmodulin-dependent protein kinase type 1, CaM kinase I, CaM-KI, CaM kinase I alpha, CaMKI-alphaCalcium/calmodulin-dependent protein kinase I is expressed in many tissues and is a component of a calmodulin-dependent protein kinase cascade. Calcium/calmodulin directly activates calcium/calmodulin-dependent protein kinase I by binding to the enzyme and indirectly promotes the phosphorylation and synergistic activation of the enzyme by calcium/calmodulin-dependent protein kinase I kinase.

Molecular Weight: 41kD

Gene ID: 8536

UniProt: Q14012

Pathways: Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Smooth Muscle Cell Migration