CAGE1 antibody (AA 680-760)

Catalog No. ABIN7213916

The Rabbit Polyclonal anti-CAGE1 antibody is suitable to detect CAGE1 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC and IF.

Quick Overview for CAGE1 antibody (AA 680-760) (ABIN7213916)

Target: CAGE1 (Cancer Antigen 1 (CAGE1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAGE1 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CAGE1 Antibody

Binding Specificity: AA 680-760

Purpose: Rabbit Anti-CAGE-1 Polyclonal Antibody

Specificity: CAGE-1 Polyclonal Antibody detects endogenous levels of CAGE-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human CAGE-1 at AA range: 680-760

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CAGE1

Target: CAGE1 (Cancer Antigen 1 (CAGE1))

Alternative Name: CAGE-1

Background: CAGE1, CTAG3, Cancer-associated gene 1 protein, CAGE-1, Cancer/testis antigen 3, CT3CAGE (cancer-associated gene protein), also known as DEAD box protien 53 (DDX53) or DEAD box protein CAGE, belongs to the DEAD box helicase family. It contains one helicase ATP-binding domain, one helicase C-terminal domain and one KH domain. CAGE localizes to the nucleus and, in normal adult tissues, is exclusively expressed in testis but it has also been found in a wide variety of cancer tissues and cell lines. Overexpression of CAGE leads to the activation of FAK, ERK and p38 MAPK along with a reduction in reactive oxygen species (ROS). It is also responsible for inducing catalase activity and therefore enhancing cell motility. This suggests that CAGE may enhance the migration of cancer cells. In addition, hypomethylation of the CAGE promoter region is associated with tumor progression and may serve as a valuable marker in cancer diagnosis.

Molecular Weight: 90kD

Gene ID: 285782

UniProt: Q8TC20