CA8 antibody (Internal Region)

Catalog No. ABIN7225002

The Rabbit Polyclonal anti-CA8 antibody (ABIN7225002) specifically detects CA8 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CA8 antibody (Internal Region) (ABIN7225002)

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CA8 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CA8 Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CA VIII Polyclonal Antibody

Specificity: CA VIII Polyclonal Antibody detects endogenous levels of CA VIII protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CA VIII

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300,WB 1:500-1:2000,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CA8

Target: CA8 (Carbonic Anhydrase VIII (CA8))

Alternative Name: CA VIII

Background: CA8, CALS, Carbonic anhydrase-related protein, CARP, Carbonic anhydrase VIII, CA-VIIIThe protein encoded by CA8 (carbonic anhydrase 8) was initially named CA-related protein because of sequence similarity to other known carbonic anhydrase genes. However, the gene product lacks carbonic anhydrase activity (i.e. The reversible hydration of carbon dioxide). The gene product continues to carry a carbonic anhydrase designation based on clear sequence identity to other members of the carbonic anhydrase gene family. The absence of CA8 gene transcription in the cerebellum of the lurcher mutant in mice with a neurologic defect suggests an important role for this acatalytic form. Mutations in CA8 are associated with cerebellar ataxia, mental retardation, and dysequilibrium syndrome 3 (CMARQ3). Polymorphisms in CA8 are associated with osteoporosis, and overexpression of CA8 in osteosarcoma cells suggests an oncogenic role. Alternative splicing results in multiple transcript variants.

Gene ID: 767

UniProt: P35219