CPE antibody (AA 240-320)

Catalog No. ABIN7214314

The Rabbit Polyclonal anti-CPE antibody is suitable to detect CPE in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CPE antibody (AA 240-320) (ABIN7214314)

Target: CPE (Carboxypeptidase E (CPE))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CPE Antibody

Binding Specificity: AA 240-320

Purpose: Rabbit Anti-CPE Polyclonal Antibody

Specificity: CPE Polyclonal Antibody detects endogenous levels of CPE protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CPE at AA range: 240-320

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CPE

Target: CPE (Carboxypeptidase E (CPE))

Alternative Name: CPE

Background: CPE, Carboxypeptidase E, CPE, Carboxypeptidase H, CPH, Enkephalin convertase, Prohormone-processing carboxypeptidaseCPE encodes a member of the M14 family of metallocarboxypeptidases. The encoded preproprotein is proteolytically processed to generate the mature peptidase. This peripheral membrane protein cleaves C-terminal amino acid residues and is involved in the biosynthesis of peptide hormones and neurotransmitters, including insulin. Carboxypeptidase E may also function independently of its peptidase activity, as a neurotrophic factor that promotes neuronal survival, and as a sorting receptor that binds to regulated secretory pathway proteins, including prohormones. Mutations in CPE are implicated in type 2 diabetes.

Molecular Weight: 53kD

Gene ID: 1363

UniProt: P16870

Pathways: Peptide Hormone Metabolism, Synaptic Membrane