CK1 epsilon antibody (AA 250-330)

Catalog No. ABIN7219756

This Rabbit Polyclonal antibody specifically detects CK1 epsilon in WB, ELISA, IHC and IF. It exhibits reactivity toward Human and Mouse.

Quick Overview for CK1 epsilon antibody (AA 250-330) (ABIN7219756)

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CK1 epsilon antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CK1 epsilon Antibody

Binding Specificity: AA 250-330

Purpose: Rabbit Anti-Casein Kinase Iε Polyclonal Antibody

Specificity: Casein Kinase Iε Polyclonal Antibody detects endogenous levels of Casein Kinase Iε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Casein Kinase Iepsilon at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CK1 epsilon

Target: CK1 epsilon (CSNK1E) (Casein Kinase 1, epsilon (CSNK1E))

Alternative Name: Casein Kinase Iepsilon

Background: CSNK1E, Casein kinase I isoform epsilon, CKI-epsilon, CKIeCasein kinase I isoform epsilon encoded by CSNK1E is a serine/threonine protein kinase and a member of the casein kinase I protein family, whose members have been implicated in the control of cytoplasmic and nuclear processes, including DNA replication and repair. The encoded protein is found in the cytoplasm as a monomer and can phosphorylate a variety of proteins, including itself. This protein has been shown to phosphorylate period, a circadian rhythm protein. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 47kD

Gene ID: 1454

UniProt: P49674

Pathways: Hedgehog Signaling, M Phase