Caspase 1 p20 antibody (Cleaved-Asn120)

Catalog No. ABIN7225262

The Rabbit Polyclonal anti-Caspase 1 p20 antibody has been validated for WB and ELISA. It is suitable to detect Caspase 1 p20 in samples from Human.

Quick Overview for Caspase 1 p20 antibody (Cleaved-Asn120) (ABIN7225262)

Target: Caspase 1 p20

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 1 p20 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 1 p20 Antibody

Binding Specificity: AA 70-150, Cleaved-Asn120

Purpose: Cleaved-Caspase-1 p20 (N120) Polyclonal Antibody

Specificity: Cleaved-Caspase-1 p20 (N120) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-1 p20 protein resulting from cleavage adjacent to N120.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human Caspase-1 p20 at AA range: 70-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for Caspase 1 p20

Target: Caspase 1 p20

Alternative Name: Caspase-1 p20

Background: Rabbit Anti-Cleaved-Caspase-1 p20 (N120) Polyclonal Antibody,CASP1, IL1BC, IL1BCE, Caspase-1, CASP-1, Interleukin-1 beta convertase, IL-1BC, Interleukin-1 beta-converting enzyme, ICE, IL-1 beta-converting enzyme, p45,CASP1 (caspase 1) encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme. CASP1 was identified by its ability to proteolytically cleave and activate the inactive precursor of interleukin-1, a cytokine involved in the processes such as inflammation, septic shock, and wound healing. CASP1 has been shown to induce cell apoptosis and may function in various developmental stages. Studies of a similar gene in mouse suggest a role in the pathogenesis of Huntington disease. Alternative splicing results in transcript variants encoding distinct isoforms.,Caspase-1

Molecular Weight: observerd band 18kDa

Gene ID: 834

UniProt: P29466