Caspase 2 p18 antibody (Cleaved-Thr325)

Catalog No. ABIN7225274

The Rabbit Polyclonal anti-Caspase 2 p18 antibody is suitable to detect Caspase 2 p18 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Caspase 2 p18 antibody (Cleaved-Thr325) (ABIN7225274)

Target: Caspase 2 p18

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 2 p18 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 2 p18 Antibody

Binding Specificity: AA 250-330, Cleaved-Thr325

Purpose: Rabbit Anti-Cleaved-Caspase-2 p18 (T325) Polyclonal Antibody

Specificity: Cleaved-Caspase-2 p18 (T325) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-2 p18 protein resulting from cleavage adjacent to T325.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Caspase-2 p18 at AA range: 250-330

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Caspase 2 p18

Target: Caspase 2 p18

Alternative Name: Caspase-2 p18

Background: CASP2, ICH1, NEDD2, Caspase-2, CASP-2, Neural precursor cell expressed developmentally down-regulated protein 2, NEDD-2, Protease ICH-1CASP2 (caspase 2) encodes a member of the cysteine-aspartic acid protease (caspase) family. Caspases mediate cellular apoptosis through the proteolytic cleavage of specific protein substrates. The encoded protein may function in stress-induced cell death pathways, cell cycle maintenance, and the suppression of tumorigenesis. Increased expression of CASP2 may play a role in neurodegenerative disorders including Alzheimer's disease, Huntington's disease and temporal lobe epilepsy. Alternatively spliced transcript variants encoding multiple isoforms have been observed for CASP2.

Molecular Weight: 22kD

Gene ID: 835

UniProt: P42575