Caspase 5 p10 antibody (Cleaved-Ser331)

Catalog No. ABIN7212442

The Rabbit Polyclonal anti-Caspase 5 p10 antibody (ABIN7212442) specifically detects Caspase 5 p10 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for Caspase 5 p10 antibody (Cleaved-Ser331) (ABIN7212442)

Target: Caspase 5 p10

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Caspase 5 p10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Caspase 5 p10 Antibody

Binding Specificity: AA 290-370, Cleaved-Ser331

Purpose: Rabbit Anti-Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody

Specificity: Cleaved-Caspase-5 p10 (S331) Polyclonal Antibody detects endogenous levels of fragment of activated Caspase-5 p10 protein resulting from cleavage adjacent to S331.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Caspase-5 p10 at AA range: 290-370

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Caspase 5 p10

Target: Caspase 5 p10

Alternative Name: Caspase-5 p10

Background: CASP5, ICH3, Caspase-5, CASP-5, ICE(rel)-III, Protease ICH-3, Protease TYCASP5 encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of caspase 5 induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by caspase 5, this process requires Fas-mediated dephosphorylation of Max. The expression of CASP5 is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for CASP5.

Molecular Weight: 10kD

Gene ID: 838

UniProt: P51878