Cathepsin L antibody (Cleaved-Thr288)

Catalog No. ABIN7212458

The Rabbit Polyclonal anti-Cathepsin L antibody is suitable to detect Cathepsin L in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for Cathepsin L antibody (Cleaved-Thr288) (ABIN7212458)

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cathepsin L antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Cathepsin L Antibody

Binding Specificity: AA 210-290, Cleaved-Thr288

Purpose: Rabbit Anti-Cleaved-Cathepsin L1 HC (T288) Polyclonal Antibody

Specificity: Cleaved-Cathepsin L1 HC (T288) Polyclonal Antibody detects endogenous levels of fragment of activated Cathepsin L1 HC protein resulting from cleavage adjacent to T288.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the C-terminal region of human Cathepsin L1 HC at AA range: 210-290

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for Cathepsin L

Target: Cathepsin L (CTSL1) (Cathepsin L1 (CTSL1))

Alternative Name: Cathepsin L1 HC

Background: CTSL1, CTSL, Cathepsin L1, Major excreted protein, MEPThe protein encoded by CTSL is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein (cathepsin L) has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. cathepsin L, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for CTSL.

Molecular Weight: 30kD

Gene ID: 1514

UniProt: P07711

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades