CEBPA antibody (pSer21)

Catalog No. ABIN7213230

The Rabbit Polyclonal anti-CEBPA antibody (ABIN7213230) specifically detects CEBPA in WB, ELISA and IHC (p). The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CEBPA antibody (pSer21) (ABIN7213230)

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPA antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CEBPA Antibody

Binding Specificity: pSer21

Purpose: C/EBP α (phospho Ser21) Polyclonal Antibody

Specificity: Phospho-C/EBP α (S21) Polyclonal Antibody detects endogenous levels of C/EBP α protein only when phosphorylated at S21.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human C/EBP alpha Phospho-Ser21

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:5000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CEBPA

Target: CEBPA (CCAAT/enhancer Binding Protein (C/EBP), alpha (CEBPA))

Alternative Name: C/EBP alpha

Background: Rabbit Anti-C/EBP α (phospho Ser21) Polyclonal Antibody,CEBPA, CCAAT/enhancer-binding protein alpha, C/EBP alpha,This intronless gene (CEBPA) encodes a transcription factor that contains a basic leucine zipper (bZIP) domain and recognizes the CCAAT motif in the promoters of target genes. The encoded protein (CCAAT/enhancer binding protein alpha) functions in homodimers and also heterodimers with CCAAT/enhancer-binding proteins beta and gamma. Activity of this protein can modulate the expression of genes involved in cell cycle regulation as well as in body weight homeostasis. Mutation of CEBPA is associated with acute myeloid leukemia. The use of alternative in-frame non-AUG (GUG) and AUG start codons results in protein isoforms with different lengths. Differential translation initiation is mediated by an out-of-frame, upstream open reading frame which is located between the GUG and the first AUG start codons.,CCAAT

Molecular Weight: observerd band 45kDa

Gene ID: 1050

UniProt: P49715

Pathways: Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation