CEBPB antibody (pThr235)

Catalog No. ABIN7212896

The Rabbit Polyclonal anti-CEBPB antibody is suitable to detect CEBPB in samples from Human, Mouse and Rat. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CEBPB antibody (pThr235) (ABIN7212896)

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPB antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CEBPB Antibody

Binding Specificity: pThr235

Purpose: Rabbit Anti-C/EBP β (phospho Thr235) Polyclonal Antibody

Specificity: Phospho-C/EBP β (T235) Polyclonal Antibody detects endogenous levels of C/EBP β protein only when phosphorylated at T235.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human C/EBP beta Phospho-Thr235

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CEBPB

Target: CEBPB (CCAAT/enhancer Binding Protein (C/EBP), beta (CEBPB))

Alternative Name: C/EBP beta

Background: CEBPB, LAP, TCF5, PP9092, CCAAT/enhancer-binding protein beta, C/EBP beta, Liver activator protein, Nuclear factor NF-IL6, Transcription factor 5, TCF-5This intronless gene (CEBPB) encodes a transcription factor (CCAAT/enhancer binding protein beta) that contains a basic leucine zipper (bZIP) domain. CCAAT/enhancer binding protein beta functions as a homodimer but can also form heterodimers with CCAAT/enhancer-binding proteins alpha, delta, and gamma. Activity of this protein is important in the regulation of genes involved in immune and inflammatory responses, among other processes. The use of alternative in-frame AUG start codons results in multiple protein isoforms, each with distinct biological functions.

Molecular Weight: 36kD

Gene ID: 1051

UniProt: P17676

Pathways: Interferon-gamma Pathway, Autophagy, Brown Fat Cell Differentiation