CEBPE antibody

Catalog No. ABIN7213872

The Rabbit Polyclonal anti-CEBPE antibody (ABIN7213872) specifically detects CEBPE in WB, ELISA, IHC and IF. The antibody is reactive with Human, Rat and Mouse samples.

Quick Overview for CEBPE antibody (ABIN7213872)

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CEBPE antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CEBPE Antibody

Purpose: Rabbit Anti-C/EBP ε Polyclonal Antibody

Specificity: C/EBP ε Polyclonal Antibody detects endogenous levels of C/EBP ε protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human C/EBP epsilon around the non-phosphorylation site of T74

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CEBPE

Target: CEBPE (CCAAT/enhancer Binding Protein (C/EBP), epsilon (CEBPE))

Alternative Name: C/EBP epsilon

Background: CEBPE, CCAAT/enhancer-binding protein epsilon, C/EBP epsilonThe protein encoded by CEBPE is a bZIP transcription factor which can bind as a homodimer to certain DNA regulatory regions. It can also form heterodimers with the related protein CEBP-delta. The encoded protein (CCAAT/enhancer binding protein epsilon) may be essential for terminal differentiation and functional maturation of committed granulocyte progenitor cells. Mutations in this gene have been associated with Specific Granule Deficiency, a rare congenital disorder. Multiple variants of this gene have been described, but the full-length nature of only one has been determined.

Molecular Weight: 34kD

Gene ID: 1053

UniProt: Q15744

Pathways: Cellular Response to Molecule of Bacterial Origin