CNOT2 antibody

Catalog No. ABIN7222790

The Rabbit Polyclonal anti-CNOT2 antibody is suitable to detect CNOT2 in samples from Human, Mouse and Rat. It has been validated for ELISA, WB, IHC and IF.

Quick Overview for CNOT2 antibody (ABIN7222790)

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CNOT2 antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CNOT2 Antibody

Purpose: Rabbit Anti-CNOT2 Polyclonal Antibody

Specificity: CNOT2 Polyclonal Antibody detects endogenous levels of CNOT2 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CNOT2 around the non-phosphorylation site of S101

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CNOT2

Target: CNOT2 (CCR4-NOT Transcription Complex, Subunit 2 (CNOT2))

Alternative Name: CNOT2

Background: CNOT2, CDC36, NOT2, HSPC131, MSTP046, CCR4-NOT transcription complex subunit 2, CCR4-associated factor 2CNOT2 encodes a subunit of the multi-component CCR4-NOT complex. The CCR4-NOT complex regulates mRNA synthesis and degradation and is also thought to be involved in mRNA splicing, transport and localization. The encoded protein interacts with histone deacetylases and functions as a repressor of polymerase II transcription. Alternatively spliced transcript variants have been observed for CNOT2.

Molecular Weight: 46kD

Gene ID: 4848

UniProt: Q9NZN8

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Stem Cell Maintenance