CD16 antibody (AA 100-150)

Catalog No. ABIN7227698

The Rabbit Polyclonal anti-CD16 antibody is suitable to detect CD16 in samples from Human and Rat. It has been validated for IF, IHC and ELISA.

Quick Overview for CD16 antibody (AA 100-150) (ABIN7227698)

Target: CD16

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD16 antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (IHC), ELISA

Product Details anti-CD16 Antibody

Binding Specificity: AA 100-150

Purpose: Rabbit Anti-CD16 Polyclonal Antibody

Specificity: The antibody detects endogenous levels of CD16

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from part region of human CD16 protein at AA range: 100-150

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:50-200,ELISA 1:10000-20000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD16

Target: CD16

Alternative Name: CD16

Background: Low affinity immunoglobulin gamma Fc region receptor III-A, CD16a antigen, Fc-gamma RIII-alpha, Fc-gamma RIII, Fc-gamma RIIIa, FcRIII, FcRIIIa, FcR-10, IgG Fc receptor III-2, CD16aFCGR3A encodes a receptor for the Fc portion of immunoglobulin G, and it is involved in the removal of antigen-antibody complexes from the circulation, as well as other other antibody-dependent responses. FCGR3A (FCGR3A) is highly similar to another nearby gene (FCGR3B) located on chromosome 1. The receptor encoded by FCGR3A is expressed on natural killer (NK) cells as an integral membrane glycoprotein anchored through a transmembrane peptide, whereas FCGR3B is expressed on polymorphonuclear neutrophils (PMN) where the receptor is anchored through a phosphatidylinositol (PI) linkage. Mutations in FCGR3A have been linked to susceptibility to recurrent viral infections, susceptibility to systemic lupus erythematosus, and alloimmune neonatal neutropenia. Alternatively spliced transcript variants encoding different isoforms have been found for FCGR3A.

Gene ID: 2214

UniProt: P08637, O75015

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process