CD3D antibody (Internal Region)

Catalog No. ABIN7214066

This Rabbit Polyclonal antibody specifically detects CD3D in WB and ELISA. It exhibits reactivity toward Human, Rat and Mouse.

Quick Overview for CD3D antibody (Internal Region) (ABIN7214066)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD3D Antibody

Binding Specificity: Internal Region

Purpose: Rabbit Anti-CD3-δ Polyclonal Antibody

Specificity: CD3-δ Polyclonal Antibody detects endogenous levels of CD3-δ protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD3-delta

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3-delta

Background: CD3D, T3D, T-cell surface glycoprotein CD3 delta chain, T-cell receptor T3 delta chain, CD antigen CD3dCD3d molecule encoded by CD3D is part of the T-cell receptor/CD3 complex (TCR/CD3 complex) and is involved in T-cell development and signal transduction. The encoded membrane protein represents the delta subunit of the CD3 complex, and along with four other CD3 subunits, binds either TCR alpha/beta or TCR gamma/delta to form the TCR/CD3 complex on the surface of T-cells. Defects in this gene are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (SCIDBNK). Two transcript variants encoding different isoforms have been found for CD3D. Other variants may also exist, but the full-length natures of their transcripts has yet to be defined.

Molecular Weight: 20kD

Gene ID: 915

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events