CD5 antibody

Catalog No. ABIN7214074

This Rabbit Polyclonal antibody specifically detects CD5 in WB and ELISA. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for CD5 antibody (ABIN7214074)

Target: CD5

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD5 Antibody

Purpose: Rabbit Anti-CD5 Polyclonal Antibody

Specificity: CD5 Polyclonal Antibody detects endogenous levels of CD5 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CD5 around the non-phosphorylation site of Y453

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:20000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD5

Target: CD5

Alternative Name: CD5

Background: CD5, LEU1, T-cell surface glycoprotein CD5, Lymphocyte antigen T1/Leu-1, CD antigen CD5CD5 (also designated Lyt-1) has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/ CD3-z chain and with the Src family kinase Lck p56. In vitro studies have shown a 10- to 15-fold increase in the kinase activity of Lck bound to CD5. The B cell antigen, CD72, serves as a receptor for CD5. The consequence of CD5 binding to its cognate receptor is still in question and likely plays a role in thymic selection.

Molecular Weight: 60kD

Gene ID: 921

UniProt: P06127