CD72 antibody (Internal Region)

Catalog No. ABIN7226672

The Rabbit Polyclonal anti-CD72 antibody (ABIN7226672) specifically detects CD72 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for CD72 antibody (Internal Region) (ABIN7226672)

Target: CD72 (CD72 Molecule (CD72))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD72 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CD72 Antibody

Binding Specificity: Internal Region

Purpose: CD72 Polyclonal Antibody

Specificity: CD72 Polyclonal Antibody detects endogenous levels of CD72 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human CD72

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CD72

Target: CD72 (CD72 Molecule (CD72))

Alternative Name: CD72

Background: Rabbit Anti-CD72 Polyclonal Antibody,CD72, B-cell differentiation antigen CD72, Lyb-2, CD72,CD5 has been identified as a transmembrane glycoprotein that is expressed on 70 % of normal peripheral blood lymphocytes and on virtually all T lymphocytes in thymus and peripheral blood. Activation of T cells through the T cell receptor (TCR) results in tyrosine phosphorylation of CD5, and the absence of CD5 renders T cells hyper-responsive to TCR-mediated activation. CD5 associates with the TCR/CD3 ζ chain, and with the Src family kinase, Lck p56. The C-type lectin superfamily member CD72 is a cell surface negative regulator of B cell activation from the pro-B through the mature B cell stage. CD72 serves as a receptor for CD5. The ability of lymphocytes to respond to antigenic or mitogenic stimulation utilizes both positive and negative regulatory proteins that influence the threshold for responsiveness. The human CD72 gene maps to chromosome 9p13.3 and encodes a transmembrane glycoprotein that contains an immunoreceptor tyrosine-based inhibition motif (ITIM). Upon tyrosine phosphorylation, the CD72 ITIM recruits SH2-containing phosphatases such as SHP-1, resulting in downregulation of cell activation. CD72-/- mice contain hyperproliferative B cells.,B-cell differentiation antigen CD72

Molecular Weight: observerd band 40kDa

Gene ID: 971

UniProt: P21854

Pathways: BCR Signaling