CD97 beta antibody (Cleaved-Ser531)

Catalog No. ABIN7226090

The Rabbit Polyclonal anti-CD97 beta antibody is suitable to detect CD97 beta in samples from Human, Mouse and Rat. It has been validated for ELISA and WB.

Quick Overview for CD97 beta antibody (Cleaved-Ser531) (ABIN7226090)

Target: CD97 beta

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD97 beta antibody is un-conjugated

Application: ELISA, Western Blotting (WB)

Product Details anti-CD97 beta Antibody

Binding Specificity: AA 480-560, Cleaved-Ser531

Purpose: Rabbit Anti-Cleaved-CD97β (S531) Polyclonal Antibody

Specificity: Cleaved-CD97β (S531) Polyclonal Antibody detects endogenous levels of fragment of activated CD97β protein resulting from cleavage adjacent to S531.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human CD97beta at AA range: 480-560

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:5000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CD97 beta

Target: CD97 beta

Alternative Name: CD97beta

Background: CD97, CD97 antigen, Leukocyte antigen CD97, CD antigen CD97ADGRE5 (adhesion G protein-coupled receptor E5) encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of ADGRE5 may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with 3 to 5 EGF-like repeats have been observed for ADGRE5. This gene is found in a cluster with other EGF-TM7 genes on the short arm of chromosome 19.

Molecular Weight: 34kD

Gene ID: 976

UniProt: P48960