CCAR2 antibody (AA 400-480)

Catalog No. ABIN7223830

The Rabbit Polyclonal anti-CCAR2 antibody is suitable to detect CCAR2 in samples from Human. It has been validated for WB, ELISA and IHC (p).

Quick Overview for CCAR2 antibody (AA 400-480) (ABIN7223830)

Target: CCAR2 (Cell Cycle and Apoptosis Regulator 2 (CCAR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCAR2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CCAR2 Antibody

Binding Specificity: AA 400-480

Purpose: DBC-1 Polyclonal Antibody

Specificity: DBC-1 Polyclonal Antibody detects endogenous levels of DBC-1 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from the Internal region of human DBC-1 at AA range: 400-480

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:20000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CCAR2

Target: CCAR2 (Cell Cycle and Apoptosis Regulator 2 (CCAR2))

Alternative Name: DBC-1

Background: Rabbit Anti-DBC-1 Polyclonal Antibody,KIAA1967, DBC1, DBIRD complex subunit KIAA1967, Deleted in breast cancer gene 1 protein, DBC-1, DBC.1, p30 DBC,Core component of the DBIRD complex, a multiprotein complex that acts at the interface between core mRNP particles and RNA polymerase II (RNAPII) and integrates transcript elongation with the regulation of alternative splicing: the DBIRD complex affects local transcript elongation rates and alternative splicing of a large set of exons embedded in (A + T)-rich DNA regions. Inhibits SIRT1 deacetylase activity leading to increasing levels of p53/TP53 acetylation and p53-mediated apoptosis. Inhibits SUV39H1 methyltransferase activity. As part of a histone H3-specific methyltransferase complex may mediate ligand-dependent transcriptional activation by nuclear hormone receptors. Plays a critical role in maintaining genomic stability and cellular integrity following UV-induced genotoxic stress. Regulates the circadian expression of the core clock components NR1D1 and ARNTL/BMAL1. Enhances the transcriptional repressor activity of NR1D1 through stabilization of NR1D1 protein levels by preventing its ubiquitination and subsequent degradation (PubMed: 18235501, PubMed: 18235502, PubMed: 19131338, PubMed: 19218236, PubMed: 22446626, PubMed: 23352644, PubMed : 23398316). Represses the ligand-dependent transcriptional activation function of ESR2 (PubMed: 20074560). Acts as a regulator of PCK1 expression and gluconeogenesis by a mechanism that involves, at least in part, both NR1D1 and SIRT1 (PubMed: 24415752). Negatively regulates the deacetylase activity of HDAC3 and can alter its subcellular localization (PubMed: 21030595). Positively regulates the beta-catenin pathway (canonical Wnt signaling pathway) and is required for MCC-mediated repression of the beta-catenin pathway (PubMed: 24824780). Represses ligand-dependent transcriptional activation function of NR1H2 and NR1H3 and inhibits the interaction of SIRT1 with NR1H3 (PubMed: 25661920). Plays an important role in tumor suppression through p53/TP53 regulation, stabilizes p53/TP53 by affecting its interaction with ubiquitin ligase MDM2 (PubMed: 25732823). Represses the transcriptional activator activity of BRCA1 (PubMed: 20160719). Inhibits SIRT1 in a CHEK2 and PSEM3-dependent manner and inhibits the activity of CHEK2 in vitro (PubMed: 25361978).,DBIRD complex subunit KIAA1967

Molecular Weight: observerd band 102kDa

Gene ID: 57805

UniProt: Q8N163