CDC25A antibody (pThr507)

Catalog No. ABIN7226154

The Rabbit Polyclonal anti-CDC25A antibody is suitable to detect CDC25A in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CDC25A antibody (pThr507) (ABIN7226154)

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25A antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CDC25A Antibody

Binding Specificity: pThr507

Purpose: Cdc25A (phospho Thr507) Polyclonal Antibody

Specificity: Phospho-Cdc25A (T507) Polyclonal Antibody detects endogenous levels of Cdc25A protein only when phosphorylated at T507.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cdc25A Phospho-Thr507

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CDC25A

Target: CDC25A (Cell Division Cycle 25 Homolog A (S. Pombe) (CDC25A))

Alternative Name: Cdc25A

Background: Rabbit Anti-Cdc25A (phospho Thr507) Polyclonal Antibody,CDC25A, M-phase inducer phosphatase 1, Dual specificity phosphatase Cdc25A,CDC25A (cell division cycle 25A) is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.,M-phase inducer phosphatase 1

Molecular Weight: observerd band 59kDa

Gene ID: 993

UniProt: P30304

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase