CDC25C antibody (pSer216)

Catalog No. ABIN7212912

This anti-CDC25C antibody is a Rabbit Polyclonal antibody detecting CDC25C in WB, ELISA and IHC (p). Suitable for Human.

Quick Overview for CDC25C antibody (pSer216) (ABIN7212912)

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC25C antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CDC25C Antibody

Binding Specificity: pSer216

Purpose: Cdc25C (phospho Ser216) Polyclonal Antibody

Specificity: Phospho-Cdc25C (S216) Polyclonal Antibody detects endogenous levels of Cdc25C protein only when phosphorylated at S216.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen

Immunogen: Synthesized peptide derived from human Cdc25C Phospho-Ser216

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB (1:500-1:2000), IHC-P (1:100-1:300), ELISA (1:10000). Not yet tested in other applications.

Comment:

Primary Antibody

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS containing 50 % Glycerol, 0.5 % BSA and 0.02 % Sodium Azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Target Details for CDC25C

Target: CDC25C (Cell Division Cycle 25 Homolog C (S. Pombe) (CDC25C))

Alternative Name: Cdc25C

Background: Rabbit Anti-Cdc25C (phospho Ser216) Polyclonal Antibody,CDC25C, M-phase inducer phosphatase 3, Dual specificity phosphatase Cdc25C,CDC25C encodes a conserved protein (cell division cycle 25C) that plays a key role in the regulation of cell division. Cell division cycle 25C directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of CDC25C have been described.,M-phase inducer phosphatase 3

Molecular Weight: observerd band 53kDa

Gene ID: 995

UniProt: P30307

Pathways: Cell Division Cycle, M Phase