CDC27 antibody (AA 330-410)

Catalog No. ABIN7214104

The Rabbit Polyclonal anti-CDC27 antibody is suitable to detect CDC27 in samples from Human, Rat and Mouse. It has been validated for WB, ELISA, IHC and IF.

Quick Overview for CDC27 antibody (AA 330-410) (ABIN7214104)

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC27 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDC27 Antibody

Binding Specificity: AA 330-410

Purpose: Rabbit Anti-Cdc27 Polyclonal Antibody

Specificity: Cdc27 Polyclonal Antibody detects endogenous levels of Cdc27 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cdc27 at AA range: 330-410

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC27

Target: CDC27 (Cell Division Cycle 27 Homolog (S. Cerevisiae) (CDC27))

Alternative Name: Cdc27

Background: CDC27, ANAPC3, D0S1430E, D17S978E, Cell division cycle protein 27 homolog, Anaphase-promoting complex subunit 3, APC3, CDC27 homolog, CDC27Hs, H-NUCThe protein encoded by CDC27 shares strong similarity with Saccharomyces cerevisiae protein Cdc27, and CDC27 product of Schizosaccharomyces pombe nuc 2. Cell division cycle 27 is a component of the anaphase-promoting complex (APC), which is composed of eight protein subunits and is highly conserved in eukaryotic cells. This complex catalyzes the formation of cyclin B-ubiquitin conjugate, which is responsible for the ubiquitin-mediated proteolysis of B-type cyclins. The protein encoded by this gene and three other members of the APC complex contain tetratricopeptide (TPR) repeats, which are important for protein-protein interactions. This protein was shown to interact with mitotic checkpoint proteins including Mad2, p55CDC and BUBR1, and it may thus be involved in controlling the timing of mitosis. Alternative splicing of this gene results in multiple transcript variants. Related pseudogenes have been identified on chromosomes 2, 22 and Y.

Molecular Weight: 92kD

Gene ID: 996

UniProt: P30260