CDC40 antibody (AA 150-230)

Catalog No. ABIN7223016

The Rabbit Polyclonal anti-CDC40 antibody (ABIN7223016) specifically detects CDC40 in WB, IHC, ELISA and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for CDC40 antibody (AA 150-230) (ABIN7223016)

Target: CDC40 (Cell Division Cycle 40 (CDC40))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC40 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CDC40 Antibody

Binding Specificity: AA 150-230

Purpose: Rabbit Anti-Cdc40 Polyclonal Antibody

Specificity: Cdc40 Polyclonal Antibody detects endogenous levels of Cdc40 protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from the Internal region of human Cdc40 at AA range: 150-230

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:40000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC40

Target: CDC40 (Cell Division Cycle 40 (CDC40))

Alternative Name: Cdc40

Background: CDC40, EHB3, PRP17, PRPF17, Pre-mRNA-processing factor 17, Cell division cycle 40 homolog, EH-binding protein 3, Ehb3, PRP17 homolog, hPRP17Pre-mRNA splicing occurs in two sequential transesterification steps. The protein encoded by CDC40 (cell division cycle 40) is found to be essential for the catalytic step II in pre-mRNA splicing process. It is found in the spliceosome, and contains seven WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp17 protein, which functions in two different cellular processes: pre-mRNA splicing and cell cycle progression. It suggests that this protein may play a role in cell cycle progression.

Molecular Weight: 66kD

Gene ID: 51362

UniProt: O60508

Pathways: Ribonucleoprotein Complex Subunit Organization