CDC6 antibody (pSer54)

Catalog No. ABIN7226142

This Rabbit Polyclonal antibody specifically detects CDC6 in WB, ELISA, IHC and IF. It exhibits reactivity toward Human, Mouse and Monkey.

Quick Overview for CDC6 antibody (pSer54) (ABIN7226142)

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Reactivity: Human, Mouse, Monkey

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CDC6 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-CDC6 Antibody

Binding Specificity: pSer54

Purpose: Rabbit Anti-Cdc6 (phospho Ser54) Polyclonal Antibody

Specificity: Phospho-Cdc6 (S54) Polyclonal Antibody detects endogenous levels of Cdc6 protein only when phosphorylated at S54.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human Cdc6 Phospho-Ser54

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,ELISA 1:5000,IF 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CDC6

Target: CDC6 (Cell Division Cycle 6 Homolog (S. Cerevisiae) (CDC6))

Alternative Name: Cdc6

Background: CDC6, CDC18L, Cell division control protein 6 homolog, CDC6-related protein, Cdc18-related protein, HsCdc18, p62(cdc6), HsCDC6The protein encoded by CDC6 (cell division cycle 6) is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. This protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins.

Molecular Weight: 62kD

Gene ID: 990

UniProt: Q99741

Pathways: DNA Replication, Synthesis of DNA