CENPA antibody

Catalog No. ABIN7219064

The Rabbit Polyclonal anti-CENPA antibody is suitable to detect CENPA in samples from Human and Mouse. It has been validated for ELISA, IF and IHC.

Quick Overview for CENPA antibody (ABIN7219064)

Target: CENPA (Centromere Protein A (CENPA))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CENPA antibody is un-conjugated

Application: ELISA, Immunofluorescence (IF), Immunohistochemistry (IHC)

Product Details anti-CENPA Antibody

Purpose: Rabbit Anti-CENP-A Polyclonal Antibody

Specificity: CENP-A Polyclonal Antibody detects endogenous levels of CENP-A protein.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CENP-A around the non-phosphorylation site of S7

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CENPA

Target: CENPA (Centromere Protein A (CENPA))

Alternative Name: CENP-A

Background: CENPA, Histone H3-like centromeric protein A, Centromere autoantigen A, Centromere protein A, CENP-ACentromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. Centromere protein A is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle. The protein is a replication-independent histone that is a member of the histone H3 family. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Gene ID: 1058

UniProt: P49450

Pathways: Chromatin Binding, Maintenance of Protein Location