CCR5 antibody (pSer336)

Catalog No. ABIN7219460

The Rabbit Polyclonal anti-CCR5 antibody is suitable to detect CCR5 in samples from Human, Mouse and Rat. It has been validated for WB and ELISA.

Quick Overview for CCR5 antibody (pSer336) (ABIN7219460)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-CCR5 Antibody

Binding Specificity: pSer336

Purpose: Rabbit Anti-CKR-5 (phospho Ser336) Polyclonal Antibody

Specificity: Phospho-CKR-5 (S336) Polyclonal Antibody detects endogenous levels of CKR-5 protein only when phosphorylated at S336.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human CKR-5 Phospho-Ser336

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CKR-5

Background: CCR5, CMKBR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD antigen CD195CCR5 encodes a member of the beta chemokine receptor family, which is predicted to be a seven transmembrane protein similar to G protein-coupled receptors. This protein is expressed by T cells and macrophages, and is known to be an important co-receptor for macrophage-tropic virus, including HIV, to enter host cells. Defective alleles of this gene have been associated with the HIV infection resistance. The ligands of this receptor include monocyte chemoattractant protein 2 (MCP-2), macrophage inflammatory protein 1 alpha (MIP-1 alpha), macrophage inflammatory protein 1 beta (MIP-1 beta) and regulated on activation normal T expressed and secreted protein (RANTES). Expression of CCR5 was also detected in a promyeloblastic cell line, suggesting that this protein may play a role in granulocyte lineage proliferation and differentiation. CCR5 is located at the chemokine receptor gene cluster region. An allelic polymorphism in this gene results in both functional and non-functional alleles, the reference genome represents the functional allele. Two transcript variants encoding the same protein have been found for this gene.

Molecular Weight: 40kD

Gene ID: 1234, 727797

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size