CXCR2 antibody (pSer347)

Catalog No. ABIN7221784

The Rabbit Polyclonal anti-CXCR2 antibody (ABIN7221784) specifically detects CXCR2 in WB, IHC, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for CXCR2 antibody (pSer347) (ABIN7221784)

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CXCR2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-CXCR2 Antibody

Binding Specificity: pSer347

Purpose: Rabbit Anti-IL-8Rβ (phospho Ser347) Polyclonal Antibody

Specificity: Phospho-IL-8Rβ (S347) Polyclonal Antibody detects endogenous levels of IL-8Rβ protein only when phosphorylated at S347.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Immunogen: Synthesized peptide derived from human IL-8Rbeta Phospho-Ser347

Isotype: IgG

Application Details

Application Notes: Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000,IHC 1:100-1:300,IF 1:200-1:1000,ELISA 1:10000,Not yet tested in other applications.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Liquid in PBS containing 50 % glycerol, 0.5 % BSA and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Expiry Date: 12 months

Target Details for CXCR2

Target: CXCR2 (Chemokine (C-X-C Motif) Receptor 2 (CXCR2))

Alternative Name: IL-8Rbeta

Background: CXCR2, IL8RB, C-X-C chemokine receptor type 2, CXC-R2, CXCR-2, CDw128b, GRO/MGSA receptor, High affinity interleukin-8 receptor B, IL-8R B, IL-8 receptor type 2, CD antigen CD182The protein encoded by CXCR2 is a member of the G-protein-coupled receptor family. This protein is a receptor for interleukin 8 (IL8). It binds to IL8 with high affinity, and transduces the signal through a G-protein activated second messenger system. This receptor also binds to chemokine (C-X-C motif) ligand 1 (CXCL1/MGSA), a protein with melanoma growth stimulating activity, and has been shown to be a major component required for serum-dependent melanoma cell growth. This receptor mediates neutrophil migration to sites of inflammation. The angiogenic effects of IL8 in intestinal microvascular endothelial cells are found to be mediated by this receptor. Knockout studies in mice suggested that this receptor controls the positioning of oligodendrocyte precursors in developing spinal cord by arresting their migration. This gene, IL8RA, a gene encoding another high affinity IL8 receptor, as well as IL8RBP, a pseudogene of IL8RB, form a gene cluster in a region mapped to chromosome 2q33-q36. Alternatively spliced variants, encoding the same protein, have been identified.

Molecular Weight: 48kD

Gene ID: 3579

UniProt: P25025

Pathways: cAMP Metabolic Process